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Acylated ghrelin inhibits high glucose-induced apoptosis in endothelial cells through PI3K/AKT pathway

Guoliang Liu

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Abstract

Objective To investigate the role of acylated ghrelin in high glucose-induced apoptosis of ECV-304 cells. Methods Western-blot and colorimetric assay were used. Results (1) Exposure of endothelial cells to acylated ghrelin (10~(-7) M)rapidly caused activation of Akt,and the effect reached the peak at 30 min. A significant difference was observed in Akt phosphorylation level between 10~(-6) M and 10~(-7) M ghrelin (P0.05),but there were no significant differences among different concentrations of acylated ghrelin (10~(-7) M, 10~(-8) M, 10~(-9) M) (P0.05). (2) The inhibition effect of acylated ghrelin on caspase-3 activition under high glucose (33.3 mM) was blocked by PI3K inhibitor (HG+G:1.051±0.014 HG+G+Inhib:1.196±0.041,P0.05).Conclusions Acylated ghrelin inhibits high glucose-induced apoptosis in endothelial cells through PI3K/Akt pathway, and the peptide may have potential in preventing diabetic complications.

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Objective To investigate the role of acylated ghrelin in high glucose-induced apoptosis of ECV-304 cells. Methods Western-blot and colorimetric assay were used. Results (1) Exposure of endothelial cells to acylated ghrelin (10~(-7) M)rapidly caused activation of Akt,and the effect reached the peak at 30 min. A significant difference was observed in Akt phosphorylation level between 10~(-6) M and 10~(-7) M ghrelin (P0.05),but there were no significant differences among different concentrations of acylated ghrelin (10~(-7) M, 10~(-8) M, 10~(-9) M) (P0.05). (2) The inhibition effect of acylated ghrelin on caspase-3 activition under high glucose (33.3 mM) was blocked by PI3K inhibitor (HG+G:1.051±0.014 HG+G+Inhib:1.196±0.041,P0.05).Conclusions Acylated ghrelin inhibits high glucose-induced apoptosis in endothelial cells through PI3K/Akt pathway, and the peptide may have potential in preventing diabetic complications.

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Available abstract

Objective To investigate the role of acylated ghrelin in high glucose-induced apoptosis of ECV-304 cells. Methods Western-blot and colorimetric assay were used. Results (1) Exposure of endothelial cells to acylated ghrelin (10~(-7) M)rapidly caused activation of Akt,and the effect reached the peak at 30 min. A significant difference was observed in Akt phosphorylation level between 10~(-6) M and 10~(-7) M ghrelin (P0.05),but there were no significant differences among different concentrations of acylated ghrelin (10~(-7) M, 10~(-8) M, 10~(-9) M) (P0.05). (2) The inhibition effect of acylated ghrelin on caspase-3 activition under high glucose (33.3 mM) was blocked by PI3K inhibitor (HG+G:1.051±0.014 HG+G+Inhib:1.196±0.041,P0.05).Conclusions Acylated ghrelin inhibits high glucose-induced apoptosis in endothelial cells through PI3K/Akt pathway, and the peptide may have potential in preventing diabetic complications.

Key concepts: Ghrelin, Protein kinase B, Apoptosis, PI3K/AKT/mTOR pathway, Internal medicine, Endocrinology, Western blot, Phosphorylation

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