Effects of Glucose and Irbesartan on Expression of Lectin-Like Oxidized Low Density Lipoprotein Receptor-1 in Cultured THP-1 Cells

Aim To investigate the influence of glucose and irbesartan on expression of lectin-like oxidized low density lipoprotein receptor-1(LOX-1) mRNA and protein in cultured THP-1 cells. Methods THP-1 cells were differentiated by incubation with 160 nmol/L PMA for 72 hours.The cells were divided into three groups: control group,glucose group(5 mmol/L,8 mmol/L,12 mmol/L,15 mmol/L),irbesartan intervention group.Then the cells in glucose group were incubated with several concentrations glucose for 24 hours. The intervention group was first treated with irbesartan for 2 h,then co-incubated with 15 mmol/L glucose for 24 h.Real time PCR and enzyme-linked immunosorbent assay technology were used to detect LOX-1 mRNA and protein expression. Results Compared with control group,the expression of LOX-1 mRNA and protein showed no significance in 5 mmol/L glucose group(P0.05),but the expression in high glucose groups increased significantly(P0.05),and the increase was dependent on high glucose concentration.Irbesartan group markedly decreased high glucose-induced LOX-1 mRNA and protein expression. Conclusion High glucose can upregulate LOX-1 mRNA and protein expression in a concentration-dependent manner and may contribute to the pathogenesis of atherosclerosis.Irbesartan can block the effect of high glucose and may produce anti-atherosclerosis.