Cloning of human CD137 molecule and its expression on COS-7 cells

Objective To clone full length human CD137 encoding sequence and to express functional CD137 molecule on the COS 7 cells. Methods PCR technique was used to clone the full length DNA encoding CD137 molecule from human T cell cDNA library. The PCR product was digested and inserted into PCI neo vector and sequenced. The right recombinant was transfected into COS 7 cell with lipofectamine reagent. The expression of CD137 molecule on the membrance of COS 7 cells was detected with FACS. Results A fragment about 800bp was cloned from human T cell cDNA library, and was subsequently digested and cloned into PCI neo vector. Sequence analysis demonstrated that the fragment cloned fits in with the published CD137 encoding sequence. FACS revealled that 27.11% cells expressed human CD137 molecules. Conclusion CD137 is cloned successfully and expresses in COS 7 cells.