2005•Di-san junyi daxue xuebaoRequires access
Recombinant of J chain-HNP-1 cDNA and the construction of expression vector
Xianhua Liu
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Abstract
Objective To construct J-HNP-1 cDNA recombinant and the eukaryotic expression vector for the reconstruction of J-HNP-1 protein which can combine with PIgR on the mucous cell membrane, penetrate into cells and kill the intracellular microorganism. Methods J chain cDNA from pCH plasmid was connected to HNP-1 cDNA from pBabeNeo plasmid by PCR, then the recombinant cDNA was inserted into the expression vector pcDNA3.1(-)/Myc-HisC. The direction and sequence in the vector was determined by sequence analysis. Results The recombinant J-HNP-1 cDNA was 786 bp and in accordance to J chain and HNP-1 cDNA of GenBank. Conclusion The recombinant J-HNP-1 cDNA and the construction of expression vector is the basis for the new bactericidal peptide production.