The Expression of ppGalNAcTs in Different Leukaemia Cells Including K562 Cell Line and SHI-1 Cell Line after 1,25(OH)_2D_3 Added

Objectives: To observe the expression of polypeptide: N-acetylgalactosaminyltransferaseTs (ppGalNAcTs) in different leukaemia cells including K562 cell line and SHI-1 cell line and to study the variation of ppGalNAcTs expression after 1,25( OH)2D3 added to the culture medium. Methods: Use RT-PCR to detect(1) the mRNA expression of ppGalNAcTs in K562 cell line and SHI-1 cell line; (2) the expression discrepancy of ppGalNAcTs in K562 cell line and SHI-1 cell line after 1,25 ( OH)2D3 added to the culture medium. Results: 1. The expression of ppGalNAc was different in two leukaemia cell lines. 2. After 1,25(OH)2D3 added to the culture medium,the mRNA expressions of ppGalNAcT2,T4,T5 in K562 cell line increased,and ppGalNAcTl in SHI-1 cell line increased too. But ppGalNAcT3 have no change,and pp-GalNAcT4 in SHI-1 cell line decreased. Conclusion: This study showed that the expression of ppGalNAcTs in different leukaemia cell was different. This result suggested that different ppGalNAcTs had different particular substances. The mRNA expression of ppGalNAcTs was defferent after 1,25(OH)2D3.