CONTRASTIVE ANALYSIS OF DETERMINATION METHOD OF SUDAN I-IV IN FOOD OF CAPSICUM

This study was established two methods based on high performance liquid chromatography(HPLC) and high performance liquid chromatography-mass spectrometry(LC-MS)for determining SudanⅠ-Ⅳin food. The SudanⅠ-Ⅳwere detected at 229 nm in HPLC. The calibration curve of SudanⅠ-Ⅳ were showed good linearity in the difference range of 0.625 ng-2 000 ng (R2=1), 2.5 ng-2 000 ng (R2=1), 5 ng-2 000 ng (R2=0.998 9) and 5 ng-2 000 ng (R2=0.9990).The detection limits of SudanⅠ-Ⅳ were 1.88 μg/kg, 2.5 μg/kg, 6.25 μg/kg and 10 μg/kg , respectively. It is applied for analysis of higher content samples .The SudanⅠ-Ⅳ were detected SIM(+) at 249.05,277.10,353.10 and 381.15(M/Z) in LC-MS ,respectively. The calibration curve of Sudan Ⅰ-Ⅲ were showed good linearity in the difference range of 0.10 ng-5 ng (R2≥0.999 7), but Sudan Ⅳ was 0.25 ng-5 ng(R2=0.999 8).The detection limits of SudanⅠ-Ⅳwere at 1 μg/kg. it is applied for analysis of microamount sample.These methods are simple and quickly.