Effects of Hydrogen Sulfide on the Expression of Tissue Factor and Tissue Factor Pathway Inhibitor Induced by ox-LDL

Aim To investigate the effects of hydrogen sulfide(H2S) on oxidized low density lipoprotein(ox-LDL) induced expression of tissue factor(TF) and tissue factor pathway inhibitor(TFPI) in endothelial cells.Methods Human umbilical vein endothelial cells(HUVEC) were cultured and treated with ox-LDL(50 mg/L) in the absence or presence of NaHS for 24 h.The mRNA expression and protein content of TF and TFPI in HUVEC were determined by RT-PCR and ELISA,respectively.Results Treatment with ox-LDL for 24 h caused an increased TF mRNA and protein expression by 8 and 7 times,respectively(P0.01),and a decreased TFPI mRNA and protein expression by 73% and 65%,respectively(P0.01).However,pretreatment of HUVEC with different concentrations(25,50,100 and 200 μmol/L) of NaHS(the donor of H2S) for 1 h before ox-LDL stimulation resulted in a marked inhibition of ox-LDL-induced TF mRNA and protein expression by 12%,31%,63%,80% and 13%,30%,62%,77%,respectively(P0.05),and an increase of ox-LDL-induced inhibition in TFPI mRNA and protein expression by 0.6,1.2,2.0,2.6 times and 0.3,0.7,1.1,1.6 times,respectively(P0.05).Conclusion H2S can inhibit ox-LDL-induced TF expression and increases ox-LDL-inhibited TFPI expression in endothelial cells.