Production of protein feed from wheat bran with solid-state fermentation by multi-strains

Yeast II with high capacity of protein synthesis in the wheat bran medium was selected from 4 strains of yeast.Yeast II and the stain of Aspergillus niger I with high cellulase activity were used for protein feed production with solid-state fermentation of multi-strains.Optimal fermentation conditions were obtained with single factor experiment and orthogonal experiment as followed: fermentation temperature 30℃,inoculum 10%,the inoculation ratio of yeast II and A.niger I 2∶1,the liquid-solid ratio 1∶1 and the glucose 2%.Under these conditions,the content of crude protein in fermentation product reached 25.26%,which was increased by 33.93% compared to that before fermentation.