Effects of sodium salicylate on the auditory brainstem response and the morphology,the expressions of γ-aminobutyricacid and glutamate of the spiral ganglion neurons in guinea pigs

Objective To observe the effects of sodium salicylate on the auditory brainstem response and the morphology,the expressions of γ-aminobutyricacid and glutamate of spiral ganglion neurons in guinea pigs.Methods Forty adult guinea pigs were randomly divided into two groups(20 cases for each) : the saline control group(group A) and the salicylate group [group B injected with a dose of 300 mg /(kg.d) for 15 days) ] respectively.After administration for 15 days,10 guinea pigs were randomly chosen,then the rest of the animals were fed without adaministration for 30 days.Auditory brainstem response(detected by Tucker Davis Technologies),scanning electron microscopy,immunohistochemistry were used to evaluate the auditory function of guinea pigs,the morphological changes and the expressions of γ-aminobutyricacid and glutamate of the spiral ganglion neurons in guinea pigs.Results After administration for 15 days,ABR thresholds of guinea pigs increased,spiral ganglion was disorganized,cell body not smooth,nerve fiber apt to divide,the expression of GABA,GLU of spiral ganglion increased in B group.After drug withdrawal for 30 days,ABR thresholds of guinea pigs was still at the high thresholds levels,spiral ganglion also exist aboved morphologically changes,the levels of GABA,GLU of spiral ganglion were higher than those after administration for 15 days(all P 0.05).But there was no obviously significance of the aboved indexes in group A.Conclusions Sodium salicylate could increase the the auditory brainstem response threshold of guinea pig and the expressions of γ-aminobutyricacid and glutamate of the spiral ganglion neurons in guinea pigs.It could also change the morphologies of the spiral ganglion neurons partly.