Nogo-A,Ng-R mRNA expression in hypoxia ischemic neonatal rat brain tissue

Aim: To observe Nogo-A,Ng-R mRNA expression in the neonatal rat brain tissue after hypoxia ischemic brain damage(HIBD). Methods:A total of 72 neonatal SD rats were allocated equally and randomly into two groups:sham operation group and HIBD group. After hypoxia treatment for 0 h,6 h,12 h,24 h,72 h,and 7 d,respectively, the rats were executed. By using RT-PCR method the mRNA expression levels of Nogo-A and Ng-R were determined. Results: Compared with sham operation group,in HIBD group,Nogo-A mRNA started to increase at 6 h and reached the peak at 12 h; Ng-R mRNA started to increase at 6 h and reached the peak at 12 h and kept increasing till 24 h.Nogo-A mRNA and Ng-R mRNA in sham operation group did not alter significantly at different time points. Conclusion: The Nogo-A and Ng-R may have certain relation with obstructing the rebirth of the nerve.