STUDY ON THE PURIFICATION OF CLONORCHIS SINENSIS ANTIGEN BY AFFINITY CHROMATOGRAPHY

Objective To explore the purification methods of the Clonorchis sinensis antigen. Methods Metocecarias were separated from the fish in clonorchiasis endemic area and infected animals. The adult worms were collected from the infection animals and then homogenates were made, in which, specification antigen were isolated by means of affinity chromatography with Sepharose 4B linked serum antibody from patients with clonorchiasis, then identified by CG-PAGE and Western blot. Results The purified antigen has 5 bands and 31 ku protein showed high antigenic, no cross reaction with Paragonimus and Schistosoma antibodies; 16 bands appeared in unpurified antigen. Conclusion Affinity chromatography is good methods for separated diagnostic antigen from the worm homogenates.