Study on freeze-drying technology of lactic acid bacteria hs-3 and ws-3

According to the relationship in freeze-drying thickness,drying time and liquid water content of freeze-drying starters,the freeze-drying time for lactic acid bacteria(LAB)strain hs-3 and ws-3 was ensured as 13 h,and the freeze-drying thickness was 0.75 cm.By screening in different cryoprotectants,the superior compound cryoprotectant for LAB freeze-drying was proved to be a mixture of 10% carrot juice,6% glucose,1% Q,1% maltodextrin and 1% W.The results indicated that the mixed freeze-drying bacterial starter of hs-3 and ws-3 had good performance in fermentation by activity determination.