Influence of Different Pigskin Degreasing Methods on Collagen Yield

The fat in the fresh pigskin was removed respectively by the following chemicals: organic solvents, nonionic degreasing agents, different amount of lipase, both nonionic degreasing agent AXAUT-50 and isopropyl alcohol, both AXAUT-50 and lipase. Then acid soluble collagen was extracted from the degreased pigskin. The results show that the degreasing effect of nonionic degreasing agents is better than that of organic solvents and lipase. The degreasing rate of nonionic degreasing agents is above 67% and the collagen yield is over 22%. And the two-step degreasing method is better than the single degreasing agent, especially the AXAUT-50 followed by isopropyl alcohol, whose degreasing rate is over 90% and the collagen yield is 28.52%.And SDS-PAGE electrophoresis analysis shows that the triple helical structure is retained in pigskin collagen, and the denaturation temperature of the collagen measured by viscosity method is 35.7℃.