The injuries of sodium citrate plasma on HepG2 cells

Objective To observe the injuries of sodium citrate plasma(scP) on the growth and function of HepG2 cells.Methods The HepG2 cells were cultured.The viability,cell cycle and apoptosis,the leakage of LDH,total protein,glutathione and the changes on morphology of hepatocytes exposured to scP were investigated.Results The viability of HepG2 cells was inhibited when the cells were cultured in scP for 24h(F=37.108,P=0.001).After 48h,nearly all cells died except cells in 10% scP group.After the cells were exposed to scP for 24h,the percentage of S phase of the cell cycle and the rate of apoptosis were significantly increased compared to those of the control.The leakages of LDH were increased in the HepG2 following exposure to scP for 5h(P0.05).Exposure of HepG2 cells to scP within 24h resulted in a decrease in the total protein synthesis and a increase in the GSH content.Conclusion scP is damaged to the growth and function of the HepG2 cells,which results from the citric acid and sodium citrate contained in anticoagulant.