Investigation of infectious status of Japanese encephalitis B virus in mosquitoes in high JE prevalence areas of Guangxi Province

Aim To isolate and identify Japanese encephalitis(JE)B virus from mosquitoes collected in high JE prevalence area of Guangxi Province.Methods Mosquitoes samples were collected in high JE B prevalence area of Guangxi Province.Japanese encephalitis B virus was isolated by incubating C6/36 and BHK21 cells with mosquito homogenizations.RT-PCR was used to identify the isolates.E genes of the virus were sequenced and genotype of the virus was analyzed by bioinformatics softwares.Results A total of 12 102 mosquitoes were collected and processed for virus isolation.Four strains of genetype Ⅰ Japanese encephalitis B virus were obtained from Culex tritaeniorhynchus collected in Beiliu and Guiping.E gene nucleic acid and amino acid homology of 4 strains was up to 98.3% and 99.8% respectively.Conclusion There 4 genetype ⅠJapanese encephalitis B virus were isolated from mosquitoes collected in Guangxi province.