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2007•The Practical Journal of CancerRequires access

RNA Interference of mdr1 Gene in Breast Cancer Cell Line MCF-7/Adr by Using the Small Interference RNA(siRNA) Synthesized with In Vitro Transcription

Yin Liu

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Abstract

Objective To explore RNA interference of mdr1 gene in breast cancer cell line MCF-7/Adr by using the small interference RNA(siRNA) synthesized with in vitro transcription.Methods The siRNA was transfected into MCF-7/Adr.For assaying the efficiency of siRNA,confocal microscopy,Northern blot and Western blot were employed to examine the expression of P-170 protein and its mRNA in MCF-7/Adr.The cellular survival rates were detected by MTT colorimetric assay.Results It was shown that the siRNA sequence-specific to mdr1 gene was capable of inhibiting the expression of P-170 protein and its mRNA.It was shown that the survival rate in siRNA group was lower compared to that in control group after cytotoxicity agent Adriamycin treatment.Conclusion Use of siRNA may be a new approach to reverse multidrug resistance(MDR).

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What this paper is about

Objective To explore RNA interference of mdr1 gene in breast cancer cell line MCF-7/Adr by using the small interference RNA(siRNA) synthesized with in vitro transcription.Methods The siRNA was transfected into MCF-7/Adr.For assaying the efficiency of siRNA,confocal microscopy,Northern blot and Western blot were employed to examine the expression of P-170 protein and its mRNA in MCF-7/Adr.The cellular survival rates were detected by MTT colorimetric assay.Results It was shown that the siRNA sequence-specific to mdr1 gene was capable of inhibiting the expression of P-170 protein and its mRNA.It was shown that the survival rate in siRNA group was lower compared to that in control group after cytotoxicity agent Adriamycin treatment.Conclusion Use of siRNA may be a new approach to reverse multidrug resistance(MDR).

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Available abstract

Objective To explore RNA interference of mdr1 gene in breast cancer cell line MCF-7/Adr by using the small interference RNA(siRNA) synthesized with in vitro transcription.Methods The siRNA was transfected into MCF-7/Adr.For assaying the efficiency of siRNA,confocal microscopy,Northern blot and Western blot were employed to examine the expression of P-170 protein and its mRNA in MCF-7/Adr.The cellular survival rates were detected by MTT colorimetric assay.Results It was shown that the siRNA sequence-specific to mdr1 gene was capable of inhibiting the expression of P-170 protein and its mRNA.It was shown that the survival rate in siRNA group was lower compared to that in control group after cytotoxicity agent Adriamycin treatment.Conclusion Use of siRNA may be a new approach to reverse multidrug resistance(MDR).

Key concepts: RNA interference, MCF-7, Small interfering RNA, Molecular biology, Transfection, Messenger RNA, RNA, Transcription (linguistics)

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RNA Interference of mdr1 Gene in Breast Cancer Cell Line MCF-7/Adr by Using the Small Interference RNA(siRNA) Synthesized with In Vitro Transcription — Research Paper | ScholarLens