Optimization of the gel-filtration chromatography parameters for separating proteins from the crude extracts of housefly

The influences of gel-filtration chromatography media, column diameter, column height and flow rate on separation performance of the protein crude extracts of housefly (Musca domestica) were investigated. The results indicated that the all above four factors could affect the separation performance of the crude extracts in gel-filtration chromatography by various degrees. In the scope of the present tests, the best separation performance was achieved using Sephadex G-75 (1.3 cm×40 cm) with 0.4 mL/min rate.