1991•Xibei Nong-Lin Keji Daxue xuebao. Ziran kexue banRequires access
Isolation and Purification of Cotton Verticillium Wilt Pathogen and the Bioassay of wilting Phytotoxin
Li Gan, Lu Jindian
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Abstract
The wilting toxin was separated and purified from Czapck's medium liquid of cotton vcrlicillium wilt pathogen by ultrafiltration and Con.A-Scpharosc 4B affinity labeling. The component analysis and the results from bioassay of wilting phytotoxin showed that toxin was a kind of glyco-protein with a strong wilting ability, which contains 14.74% of carbonhydratc and 85.26% of protein .The toxin wilting activity was 12-15 times higher than that of half crude toxin , and the retraction wilting activity was 76%.