AZF Microdeletion Analysis of Test Results in 215 Male Patients with Infertility

Objective:To study the significance of the Y chromosome azoospermia factor AZF(azoospermia factor)for the detection of gene diagnosis of male infertility causes of importent.Methods:268 patients were tested for the six reproductive hormone testing,semen detection and conventional karyotype analysis.215 normal karyotypes were selected according to the results of the analysis and were divided into four groups:azoospermia group,serious oligospermatism group,oligospermia group and other groups(including recurrent spontaneous abortion and cryptorchidism or severe varicocele patients).Multi-PCR technology was used in testing AZFgenes.Results:In 215 patients,15 cases showed different sites of AZF gene deletion;6 depetion in azoospermia group(14.6%),9 in serious oligospermia(13.8%)and no depletion was found in the other groups.The depletion rates of azoospermia and severe oligospermia groups had significant difference when compared with that of the other groups(P0.05).Conclusion:Y chromosome microdeletions germ is one of the important reasons of serious importence.AZF gene screening is a simple and effective primary diagnosis of azoospermia and severe oligospermia.