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A Study on Mycorrhization of Pines with Vegetative Inoculum of Pisolithus tinctorius in Nursery

Caf Beijing

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Abstract

The success of the artificial inoculation of pines with the vegetative inoculumof Pisolithus tinctorius (Pt ) is confirmed by means of making a comparison of cultures be-tween reisolates from ectomycorrhizae formed and mother isolate inoculated ,and an identifi-cation of sporocarps connected with the ectomycorrhizae;the gross form and anatomy struc-ture of the ectomycorrhizae formed after inoculation are described by using the photos fromthe experiment. It is discovered that the typical ectomycorrhizae fungus , Pt can form ecten-domycorrhizae with pines under the condition of artificial inoculation in nursery.The application experiments conducted in nurseries give evidence that inoculum made bythe Center for Mycorrhizal Research and Development,CAF is effective in increasing ectomy-corrhizae,promoting the growth and improving the biomass of the seedlings,100%seedlingsof four pines receiving vegetative Ptinoculum have got Pt ectomycorrhizae with Pt index 88+,the yield of plantable seedlings increased by more than 14. 6%,and height,root-collar di-ameter, biomass, the number and the total length of the lateral root for the ten-month-oldseedlings increased,on the average,by 28.1%~71.4%, 22.8%~49.2%, 149.2%~457.1%,150.0%~200.0%and 82.4%~101.1%respectively.

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The success of the artificial inoculation of pines with the vegetative inoculumof Pisolithus tinctorius (Pt ) is confirmed by means of making a comparison of cultures be-tween reisolates from ectomycorrhizae formed and mother isolate inoculated ,and an identifi-cation of sporocarps connected with the ectomycorrhizae;the gross form and anatomy struc-ture of the ectomycorrhizae formed after inoculation are described by using the photos fromthe experiment. It is discovered that the typical ectomycorrhizae fungus , Pt can form ecten-domycorrhizae with pines under the condition of artificial inoculation in nursery.The application experiments conducted in nurseries give evidence that inoculum made bythe Center for Mycorrhizal Research and Development,CAF is effective in increasing ectomy-corrhizae,promoting the growth and improving the biomass of the seedlings,100%seedlingsof four pines receiving vegetative Ptinoculum have got Pt ectomycorrhizae with Pt index 88+,the yield of plantable seedlings increased by more than 14. 6%,and height,root-collar di-ameter, biomass, the number and the total length of the lateral root for the ten-month-oldseedlings increased,on the average,by 28.1%~71.4%, 22.8%~49.2%, 149.2%~457.1%,150.0%~200.0%and 82.4%~101.1%respectively.

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Available abstract

The success of the artificial inoculation of pines with the vegetative inoculumof Pisolithus tinctorius (Pt ) is confirmed by means of making a comparison of cultures be-tween reisolates from ectomycorrhizae formed and mother isolate inoculated ,and an identifi-cation of sporocarps connected with the ectomycorrhizae;the gross form and anatomy struc-ture of the ectomycorrhizae formed after inoculation are described by using the photos fromthe experiment. It is discovered that the typical ectomycorrhizae fungus , Pt can form ecten-domycorrhizae with pines under the condition of artificial inoculation in nursery.The application experiments conducted in nurseries give evidence that inoculum made bythe Center for Mycorrhizal Research and Development,CAF is effective in increasing ectomy-corrhizae,promoting the growth and improving the biomass of the seedlings,100%seedlingsof four pines receiving vegetative Ptinoculum have got Pt ectomycorrhizae with Pt index 88+,the yield of plantable seedlings increased by more than 14. 6%,and height,root-collar di-ameter, biomass, the number and the total length of the lateral root for the ten-month-oldseedlings increased,on the average,by 28.1%~71.4%, 22.8%~49.2%, 149.2%~457.1%,150.0%~200.0%and 82.4%~101.1%respectively.

Key concepts: Ectomycorrhizae, Pisolithus, Inoculation, Biology, Mycorrhiza, Botany, Horticulture, Biomass (ecology)

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