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Effects of icariin on the expression ofβ-actin,TRAP and RANK mRNA in mouse osteoclasts

Xingyan Liu

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Abstract

Objective To observe the effects of icariin on the expression ofβ-actin,TRAP and RANK mRNA in mouse osteoclasts cultured in vitro.Methods Osteoclasts were obtained from mouse bone marrow induced by M-CSF(25μg/L),RANKL(30μg/L)and 1,25(OH)2VitD3(1×10~(-8)mol/ L).After treatment with different concentrations of icariin(0 mol/L,1×10~(-5)mol/L)for 48 h,total RNA was isolated from osteoclasts.The expression ofβ-actin,TRAP and RANK mRNA was detected by semi- quantitative RT-PCR.Results Compared with the control group,icariin(1×10~(-5)mol/L)remarkably decreasedβ-actin and RANK mRNA levels(both P0.05),but had no significant effects on the expres- sion of TRAP mRNA(P0.05).Conclusion Icariin could inhibit inducing osteoclast formation and bone resorption by downregulareing the expression ofβ-actin and RANK mRNA.

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Objective To observe the effects of icariin on the expression ofβ-actin,TRAP and RANK mRNA in mouse osteoclasts cultured in vitro.Methods Osteoclasts were obtained from mouse bone marrow induced by M-CSF(25μg/L),RANKL(30μg/L)and 1,25(OH)2VitD3(1×10~(-8)mol/ L).After treatment with different concentrations of icariin(0 mol/L,1×10~(-5)mol/L)for 48 h,total RNA was isolated from osteoclasts.The expression ofβ-actin,TRAP and RANK mRNA was detected by semi- quantitative RT-PCR.Results Compared with the control group,icariin(1×10~(-5)mol/L)remarkably decreasedβ-actin and RANK mRNA levels(both P0.05),but had no significant effects on the expres- sion of TRAP mRNA(P0.05).Conclusion Icariin could inhibit inducing osteoclast formation and bone resorption by downregulareing the expression ofβ-actin and RANK mRNA.

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Available abstract

Objective To observe the effects of icariin on the expression ofβ-actin,TRAP and RANK mRNA in mouse osteoclasts cultured in vitro.Methods Osteoclasts were obtained from mouse bone marrow induced by M-CSF(25μg/L),RANKL(30μg/L)and 1,25(OH)2VitD3(1×10~(-8)mol/ L).After treatment with different concentrations of icariin(0 mol/L,1×10~(-5)mol/L)for 48 h,total RNA was isolated from osteoclasts.The expression ofβ-actin,TRAP and RANK mRNA was detected by semi- quantitative RT-PCR.Results Compared with the control group,icariin(1×10~(-5)mol/L)remarkably decreasedβ-actin and RANK mRNA levels(both P0.05),but had no significant effects on the expres- sion of TRAP mRNA(P0.05).Conclusion Icariin could inhibit inducing osteoclast formation and bone resorption by downregulareing the expression ofβ-actin and RANK mRNA.

Key concepts: Icariin, Messenger RNA, Bone resorption, RANKL, Chemistry, Osteoclast, In vitro, Molecular biology

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Effects of icariin on the expression ofβ-actin,TRAP and RANK mRNA in mouse osteoclasts — Research Paper | ScholarLens