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2006•Unpublished venueRequires access

Application of PCR-DGGE to Study Microbial Diversity in Glacier No.1 Soils in Xinjiang

Peng Shi

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Abstract

Separation of polymerase chain reaction (PCR)-amplified 16S rDNA products using denaturing gradient gel electrophoresis(DGGE) was tested as a means to study microbial community composition in soil samples.The soil total DNA was directly extracted from five Glacier No.1 soils from different altitudes in xinjiang.Two sets of primers specific for bacteria (V3 and V6/V9 regions of 16S rDNA) were used to amplify specific segments.The PCR products were analyzed using DGGE.The electrophoresis lanes obtained by PCR-DGGE were legible and distinguishable.The results suggested that PCR-DGGE is a rapid and efficient method to discriminate among microbial communities.

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What this paper is about

Separation of polymerase chain reaction (PCR)-amplified 16S rDNA products using denaturing gradient gel electrophoresis(DGGE) was tested as a means to study microbial community composition in soil samples.The soil total DNA was directly extracted from five Glacier No.1 soils from different altitudes in xinjiang.Two sets of primers specific for bacteria (V3 and V6/V9 regions of 16S rDNA) were used to amplify specific segments.The PCR products were analyzed using DGGE.The electrophoresis lanes obtained by PCR-DGGE were legible and distinguishable.The results suggested that PCR-DGGE is a rapid and efficient method to discriminate among microbial communities.

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Available abstract

Separation of polymerase chain reaction (PCR)-amplified 16S rDNA products using denaturing gradient gel electrophoresis(DGGE) was tested as a means to study microbial community composition in soil samples.The soil total DNA was directly extracted from five Glacier No.1 soils from different altitudes in xinjiang.Two sets of primers specific for bacteria (V3 and V6/V9 regions of 16S rDNA) were used to amplify specific segments.The PCR products were analyzed using DGGE.The electrophoresis lanes obtained by PCR-DGGE were legible and distinguishable.The results suggested that PCR-DGGE is a rapid and efficient method to discriminate among microbial communities.

Key concepts: Temperature gradient gel electrophoresis, 16S ribosomal RNA, Biology, Polymerase chain reaction, Soil water, Microbial population biology, Ribosomal DNA, Bacteria

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Application of PCR-DGGE to Study Microbial Diversity in Glacier No.1 Soils in Xinjiang — Research Paper | ScholarLens