Constituents Analysis of Cortex Magnoliae Officinalis Supercritical Fluid Extraction by UPLC/Q-TOF-MS in vitro and in vivo

Objective To analyze the consitituents of Cortex Magnoliae Officinalis supercritical fluid extraction(SFE) in vitro and in vivo by using ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry(UPLC/Q-TOF-MS).Methods The chromatographic separation was performed on a ACQUITY UPLC BEH C18 column with a gradient elution of water(A)-acetonitrile(B).The mass spectrometer equipped with electrospray ionization source was used as the detector and was operated under the negative ion modes.The identification of the chemical composition of extracts was performed and metabolism of compound absorbed into blood was also investigated preliminarily.Results Of the 5 original compounds detected,magnolol was absorbed into blood and its 3 metabolites were preliminarily identified.Conclusion Magnolol may be the bioactive component of Cortex Magnoliae Officinalis SFE in vivo.The analysis of its metabolite may lay a foundation for the exploration of the active ingredients of Cortex Magnoliae Officinalis SFE.