2007•Journal of Analytical ScienceRequires access

Se-polysaccharides Speciation in Flammulina Velutipes by SE-HPLC-ICP-MS

Shuliang Zang

Abstract

Se-polysaccharides in which the Se concentration is 7.97 μg/g were extracted in Se-enriched Flammulina Velutipes.SE-HPLC-ICP-MS hyphenated technique was used to separate and investigate the speciation of Se-polysaccharides.Three types of polysaccharides containing Se were found in Se-enriched Flammulina Velutipes,and their molecular weight were 4.27 kDa,1.868 kDa and 1.54 kDa,respectively.Se content in above Se-polysaccharides accounted for 14.95%,40.39%,44.66% of total Se in soluble Se-polysaccharides.It indicated that Se participated in the synthesis of hiomacro molecules and inorganic Se could be turned into organic Se with bioactivity in the growth of Flammulina Velutipes.Therefore,Flammulina Velutipes is possible to become a new Se source.

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Key concepts: Flammulina, Chemistry, Polysaccharide, Food science, High-performance liquid chromatography, Chromatography, Biochemistry, Mushroom