Determination of cyromazine residues in chicken tissues by high-performance liquid chromatography

A method was developed for the determination of cyromazine in chicken muscle and liver by high-performance liquid chromatography. Cyromazine was extracted from tissue samples with acetonitrile, and cleared up by anion exchange chromatography. Cyromazine was determined by HPLC in a single analysis on NH2 column with Uv detection at 214 nm, and mobile phase was acetonitrile-water (95 + 5). The limit of detection was 5 ng · kg-1. The average recoveries of cyromazine spiked to tissue were (69. 97±4.84)% , (71. 26±4. 43) % and (79. 40± 1. 05) % for liver samples respectively, and (71. 85± 4. 81)% , (73. 18± 4. 27)% and (81. 16 ±3. 86)% for muscle samples respectively when fortified with 0. 02, 0. 05 and 0. 20 mg·kg-1. The response for cyromazine was linear in the range of 0. 01- 1. 00 mg· kg-1(r = 0. 999 7, n=7). The within-day coefficient of variation (CV) and between-day CV were less than 8.69% and 10.05% respectively. The method can meet the demand of residue determination of cyromazine in chicken tissues.