Effect of drynaria-containing serum on the proliferation and osteogenesis of osteoblasts

Objective To investigate the effect of drynaria-containing serum on the proliferation and osteogenesis of osteoblasts. Methods The cranium of the suckling mice were collected,then digested with type II collagenase repeatedly,and then centrifuged,in order to extract osteoblasts. The intervention of drynaria-containing serum was applied. Then,the proliferation rate of cells,the ALP activity,the amount of calcium deposition,and the secretion of osteocalcin were determined respectively,in order to investigate the effect of drynaria-containing serum on the proliferation,the osteogenic differentiation,and the antioxidant activity of osteoblasts. Results After the intervention of drynaria-containing serum,the proliferation rate,the ALP activity,the amount of calcium deposition,the secretion of osteocalcin,and the calcified nodules were higher than that in blank control group. Conclusion The drynaria-containing serum can promote the proliferation and osteogenic differentiation of osteoblasts,and enhance its antioxidant activity.