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Study on the fermentation of the xylanase by Bacillus sp.B_(50)

Niu Tian-gui

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Abstract

The fermentation conditions of xylanase by Bacillus sp. B50 are optimized in the study through the single factor and the orthogonal experiment. So the best conditions are: carbon source: 5% bran, nitrogen source: 1% (NH4)2HPO4, yeast 0.2%, NaCl 0.6%, K2HPO4 0.2%, CaCl2 0.01%, FeSO4 0.01%, MgSO4 0.01%, start pH7.0, the inoculation content 5%, 20mL in 100mL triangular flask, the best temperature is 25℃ and the time is 60h. So the xylanase activity can reach about 15362.6U/mL.

About this research paper

What this paper is about

The fermentation conditions of xylanase by Bacillus sp. B50 are optimized in the study through the single factor and the orthogonal experiment. So the best conditions are: carbon source: 5% bran, nitrogen source: 1% (NH4)2HPO4, yeast 0.2%, NaCl 0.6%, K2HPO4 0.2%, CaCl2 0.01%, FeSO4 0.01%, MgSO4 0.01%, start pH7.0, the inoculation content 5%, 20mL in 100mL triangular flask, the best temperature is 25℃ and the time is 60h. So the xylanase activity can reach about 15362.6U/mL.

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Available abstract

The fermentation conditions of xylanase by Bacillus sp. B50 are optimized in the study through the single factor and the orthogonal experiment. So the best conditions are: carbon source: 5% bran, nitrogen source: 1% (NH4)2HPO4, yeast 0.2%, NaCl 0.6%, K2HPO4 0.2%, CaCl2 0.01%, FeSO4 0.01%, MgSO4 0.01%, start pH7.0, the inoculation content 5%, 20mL in 100mL triangular flask, the best temperature is 25℃ and the time is 60h. So the xylanase activity can reach about 15362.6U/mL.

Key concepts: Xylanase, Bran, Food science, Fermentation, Carbon source, Nitrogen, Inoculation, Bacillus (shape)

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