2011China Animal Husbandry & Veterinary MedicineRequires access

Genetic Diversity of Microsatellite DNA in Nine Sheep Breeds

Xu Han

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Abstract

The genetic diversity of nine Chinese indigenous sheep breeds were analyzed with fluorescence PCR using 10 microsatellite markers recommended by the FAO and ISGA.The results showed that 153 alleles were detected on 10 microsatellite loci,the effective number of which ranged from 2.3064 to 13.7632,and the loci polymorphism information content and heterozygosity varied from 0.5395 to 0.8559 and from 0.3972 to 0.8475,with locus genetic differentiation in the range from 0.042 to 0.149.The conclusion of the genetic structure relationship and DA / UPGMA dendrogram and MVSP cluster analysis diagram was basically consistent with sheep origin and evolution and genetic relationship and geographic distribution.

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What this paper is about

The genetic diversity of nine Chinese indigenous sheep breeds were analyzed with fluorescence PCR using 10 microsatellite markers recommended by the FAO and ISGA.The results showed that 153 alleles were detected on 10 microsatellite loci,the effective number of which ranged from 2.3064 to 13.7632,and the loci polymorphism information content and heterozygosity varied from 0.5395 to 0.8559 and from 0.3972 to 0.8475,with locus genetic differentiation in the range from 0.042 to 0.149.The conclusion of the genetic structure relationship and DA / UPGMA dendrogram and MVSP cluster analysis diagram was basically consistent with sheep origin and evolution and genetic relationship and geographic distribution.

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Available abstract

The genetic diversity of nine Chinese indigenous sheep breeds were analyzed with fluorescence PCR using 10 microsatellite markers recommended by the FAO and ISGA.The results showed that 153 alleles were detected on 10 microsatellite loci,the effective number of which ranged from 2.3064 to 13.7632,and the loci polymorphism information content and heterozygosity varied from 0.5395 to 0.8559 and from 0.3972 to 0.8475,with locus genetic differentiation in the range from 0.042 to 0.149.The conclusion of the genetic structure relationship and DA / UPGMA dendrogram and MVSP cluster analysis diagram was basically consistent with sheep origin and evolution and genetic relationship and geographic distribution.

Key concepts: Microsatellite, Genetic diversity, UPGMA, Biology, Dendrogram, Locus (genetics), Genetic relationship, Loss of heterozygosity

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