STUDY ON THE DEGRADATION OF LACTOSE IN THE MILK BY ASPERGILLUS ORYZAE

Using the milk as testing material,degradation of lactose by the fermentation of Aspergillus oryzae,the cultural conditions were:inoculation size 3%,at a temperature of 28 ℃±1 ℃,medium volume 30 mL/250 mL,shake flask,180 r/min and fermentation 60 h.Under these conditions,the hydrolysis rate of lactase form fermentation can reach on 31.4 %;through the degradation of crude extract of Aspergillus oryzae,hydrolysis rate of lactose can reach on 41.4%～55.0%.The composition of milk lactose by fermentation using thin-layer chromatography analysis,but also explain biodegradable lactose.