Inhibitory effects of siRNA on MDM2 expression and cell proliferation in osteosarcoma cell line

Objective To study the inhibitory effects of siRNA on MDM2 expression in osteosarcoma cell line U20S and cell proliferation.Methods siRNA targeting MDM2(PGCsilencerTM-MDM2-siRNA) was constructed and transfercted into U20S cells.Meanwhile,control siRNA was transfected and the transfection agent was used as the comparison.MDM2 gene and protein expressions were measured using RT-PCR and Western blotting.Cell proliferation was measured with MTT assay.Results RT-PCR and Western blotting results showed that MDM2 mRNA expression levels were reduced to 32.61% and 39.06% after being transfected with siMDM2-1 and siMDM2-2; MDM2 protein expression levels decreased to 35.76% and 42.20%,respectively.The difference between control siRNA and liposome group was not obvious(P0.05).MTT result showed that the cell growth was inhibited by being transfected with siMDM2,the difference was obvious compared with liposome group(P0.05).The difference was not significant between control siRNA and liposome group (P0.05).Conclusion siRNA can effectively down-regulate MDM2 expression in U20S cell line and inhibit the cell proliferation.