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2016•Emerging infectious diseasesOpen access

Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses

Jesse J. Waggoner, Lionel Gresh, Alisha Mohamed-Hadley, Gabriela Ballesteros, Maria Jose Vargas Davila, Yolanda Téllez, Malaya K. Sahoo, Ángel Balmaseda, Eva Harris, Benjamin A. Pinsky

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Abstract

Clinical manifestations of Zika virus, chikungunya virus, and dengue virus infections can be similar. To improve virus detection, streamline molecular workflow, and decrease test costs, we developed and evaluated a multiplex real-time reverse transcription PCR for these viruses.

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What this paper is about

Clinical manifestations of Zika virus, chikungunya virus, and dengue virus infections can be similar. To improve virus detection, streamline molecular workflow, and decrease test costs, we developed and evaluated a multiplex real-time reverse transcription PCR for these viruses.

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OpenAlex reports 218 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

Clinical manifestations of Zika virus, chikungunya virus, and dengue virus infections can be similar. To improve virus detection, streamline molecular workflow, and decrease test costs, we developed and evaluated a multiplex real-time reverse transcription PCR for these viruses.

Key concepts: Chikungunya, Zika virus, Virology, Dengue fever, Multiplex, Dengue virus, Biology, Virus

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