Comparison of the detection and quantification of rabies antibodies in canine sera
Anna Ondrejková, Judit Süli, R. Ondrejka, Z. Beníšek, Richard Franka, Štefan Švrček, Marián Maďar, Andrej Bugarský
Abstract
Anna Ondrejková, Judit Süli, R. Ondrejka, Z. Beníšek, Richard Franka, Štefan Švrček, Marián Maďar, Andrej Bugarský
Abstract
Rabies antibodies in canine sera were detected and quantified by methods: virus neutralisation test on mice (VNT), rapid fluorescence focus inhibition test (RFFIT), and fluorescent antibody virus neutralisation (FAVN). The results of rabies antibodies levels in non-vaccinated dogs obtained by all three methods were in correlation. The comparison of rabies antibody titres determined in vaccinated dogs using VNT and FAVN methods showed 86.6% correspondence, while those obtained by RFFIT and FAVN methods corresponded in almost 95% of cases.
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Rabies antibodies in canine sera were detected and quantified by methods: virus neutralisation test on mice (VNT), rapid fluorescence focus inhibition test (RFFIT), and fluorescent antibody virus neutralisation (FAVN). The results of rabies antibodies levels in non-vaccinated dogs obtained by all three methods were in correlation. The comparison of rabies antibody titres determined in vaccinated dogs using VNT and FAVN methods showed 86.6% correspondence, while those obtained by RFFIT and FAVN methods corresponded in almost 95% of cases.
Key concepts: Rabies virus, Rabies, Virology, Antibody, Neutralization, Lyssavirus, Medicine, Virus