Evaluation of Immunostimulant and Vaccine on Labeo rohita (Rohu)

Immunostimulant is used in combination with formalin inactivated vaccines which gives early activation to non-specific defense mechanisms. However, it also elevates the specific immune response and enhances protection against bacterial infection in fish. The present study demonstrates an effect of immunostimulant viz. vitamin C along with formalin inactivated polyvalent vaccine on fingerlings of Labeo rohita (rohu). The statistical significance of difference in the number of survivals in four vaccines and vitamin C groups, for before and after challenge was obtained using Fisher’s exact test. The relative percent survival (RPS) was found to be higher for the group treated with immunostimulant vitamin C supplement; while lower for group treated with monovalent and polyvalent without vitamin C. The difference in the proportion of survivals in vaccine (T4/T5/T6) and control group after challenge was found statistically significant at 36th day.