Establishment of Agrobacterium rhizogenes-mediated Hairy Roots Inducement System for Mulberry

Using direct infection and co-culturing with bacteria method,10-days mulberry cotyledon were wound-inoculated with Agrobacterium rhizogenes strains ACCC10060 in order to obtain hairy roots.Explants were cultured in dark 2 days on the MS+AS(Acetosyringone,100 μmol/L),then transferred onto MS+CS(Cefotaxim sodium,200 mg/L) in order to cure the bacteria,and replaced every 3 days for 3 weeks.Both infection methods were successfully obtained mulberry hairy roots,and the rate of induction are 14% and 17%,respectively.The root grew vigorously on MS medium without any hormone and has hairy root characters.Genomic DNA of the hairy roots was extracted by CTAB method and a 423 bp fragment was amplified with primers of rolB,indicated that T-DNA of Ri had integrated into mulberry genome.