Antioxidant Activity Of Alstonia Scholarsis Extracts Containing Flavonoid And Phenolic Compounds

The present work is carried out to screen for total phenols, flavonoids, and free radical scavenging activity in methanolic extract of leaf, root and bark of Alstonia scholaris Linn (Apocynaceae) using in vitro tests including 1, 1-diphenyl-2-picryl-hydrazil (DPPH) free radical scavenging and superoxide anion radical scavenging methods. Significant differences in DPPH scavenging activity were found between methanolic extract of leaf, root and bark were investigated; the highest radical scavenging activity were observed in root extract (33.00±4.62% inhibition). The total phenol content of the investigated results ranged from 34.97± 0.76 to 46.11 ± 0.85mg GAE/g extract; while flavonoid content ranged from 14.43± 2.37 to 22.54 ± 0.98 mg QE/g extract and the antioxidant activity of the methanol extract increased in a concentration-dependent manner.