Fully Automated Compound Screening in Arabidopsis thaliana Seedlings
Dominique Audenaert, Long Nguyen, Bert De Rybel, Tom Beeckman
Abstract
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Dominique Audenaert, Long Nguyen, Bert De Rybel, Tom Beeckman
Abstract
Open-access reader
High-throughput small molecule screenings in model plants are of great value to identify compounds that interfere with plant developmental processes. In academic research, the plant Arabidopsis thaliana is the most commonly used model organism for this purpose. However, compared to plant cellular systems, A. thaliana plants are less amenable to develop high-throughput screening assays. In this chapter, we describe a screening procedure that is compatible with liquid handling systems and increases the throughput of compound screenings in A. thaliana seedlings.
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High-throughput small molecule screenings in model plants are of great value to identify compounds that interfere with plant developmental processes. In academic research, the plant Arabidopsis thaliana is the most commonly used model organism for this purpose. However, compared to plant cellular systems, A. thaliana plants are less amenable to develop high-throughput screening assays. In this chapter, we describe a screening procedure that is compatible with liquid handling systems and increases the throughput of compound screenings in A. thaliana seedlings.
Key concepts: Arabidopsis thaliana, High-throughput screening, Throughput, Organism, Biology, Computational biology, Plant species, Botany