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2015•Asian Journal of Pharmacy and TechnologyOpen access

Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Irbesartan and Atorvastatin in Synthetic Mixture

Virani Paras, Rajanit Sojitra, Bhadresh Savaj, Raj Hashumati, Jain Vineet, Patel Kalpesh, Vishnu Sutariya

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Abstract

A simple, accurate, rapid and precise reverse phase high performance liquid chromatographic method was developed for simultaneous estimation of Irbesartan and Atorvastatin in synthetic mixture. Inertsil C18, 150 mm x 4.6 mm, 5 μm particle size in gradient mode with mobile phase Acetonitrile: 0.1% Formic acid (40: 60 v/v) and pH adjusted to 3.5 ± 0.1 with orthophosphoric acid was used. The flow rate was 1.0 ml/min and absorbance of individual component was measured at 262 nm. The retention times of Irbesartan and Atorvastatin were found to be 3.993 and 7.733 min, respectively. Linearity for Irbesartan and Atorvastatin was in the range of 400 -800 and 50–100μg/ml with correlation coefficient values 0.9995and 0.9994, the percentage recovery obtained was 99.88and 99.70%, respectively.

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A simple, accurate, rapid and precise reverse phase high performance liquid chromatographic method was developed for simultaneous estimation of Irbesartan and Atorvastatin in synthetic mixture. Inertsil C18, 150 mm x 4.6 mm, 5 μm particle size in gradient mode with mobile phase Acetonitrile: 0.1% Formic acid (40: 60 v/v) and pH adjusted to 3.5 ± 0.1 with orthophosphoric acid was used. The flow rate was 1.0 ml/min and absorbance of individual component was measured at 262 nm. The retention times of Irbesartan and Atorvastatin were found to be 3.993 and 7.733 min, respectively. Linearity for Irbesartan and Atorvastatin was in the range of 400 -800 and 50–100μg/ml with correlation coefficient values 0.9995and 0.9994, the percentage recovery obtained was 99.88and 99.70%, respectively.

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Available abstract

A simple, accurate, rapid and precise reverse phase high performance liquid chromatographic method was developed for simultaneous estimation of Irbesartan and Atorvastatin in synthetic mixture. Inertsil C18, 150 mm x 4.6 mm, 5 μm particle size in gradient mode with mobile phase Acetonitrile: 0.1% Formic acid (40: 60 v/v) and pH adjusted to 3.5 ± 0.1 with orthophosphoric acid was used. The flow rate was 1.0 ml/min and absorbance of individual component was measured at 262 nm. The retention times of Irbesartan and Atorvastatin were found to be 3.993 and 7.733 min, respectively. Linearity for Irbesartan and Atorvastatin was in the range of 400 -800 and 50–100μg/ml with correlation coefficient values 0.9995and 0.9994, the percentage recovery obtained was 99.88and 99.70%, respectively.

Key concepts: Irbesartan, Atorvastatin, Chromatography, Formic acid, Chemistry, Correlation coefficient, Retention time, Dosage form

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Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Irbesartan and Atorvastatin in Synthetic Mixture — Research Paper | ScholarLens