Studies on lytic enzyme against cariogenic streptococci. III. Purification and properties of lytic enzymes from Streptomyces globisporus 1829.

Two lytic enzymes capable of lysing Streptococcus mutans have been purified to give a single band on disc-gel electrophoresis, respectively.The M-1 and M-2 enzymes were both proved to be N-acetylmuramidases.However, these enzymes were entirely different on their enzymatic properties.The molecular weights were about 20,000 and 11,000 for M-1 and M-2 enzymes, respectively, The maximal lytic activity of M-1 enzyme was obtained at ionic strength 0.05, while lytic activity of M-2 enzyme did not change within the ionic strength range of 0 to 0.05.The M-1 enzyme constituted the majority of the total lytic activity against the cell walls of Streptococcus mutans BHT of cultured filtrate.The M-2 enzyme showed less specific lytic activity on the cell walls of Streptococcus mutans BHT than M-1 enzyme.