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2014•Journal of medical research/˜The œjournal of medical researchRequires access

Autochthonous Cases of Dirofilaria in Dogs from TimiÈ™ County (Western Part) Romania

R. Ciocan, N. Mederle, Olga JacsÃ, Balázs Tánczos, Éva Fok

Open publisher page 6 citations

Abstract

Background: Taking into consideration that dirofilariosis spreading in Romania, in this study we intended to determine the prevalence of Dirofilaria spp. infection in dogs from Timiș County. Methods: The epidemiological research was conducted between February 2008 and October 2010, on a total of 457 dogs. The modified Knott's method was used to identify the Dirofilaria spp. microfilariae. Blood samples positive for microfilariae were examined using the polymerase chain reaction (PCR), at the Szent Istvan University, Faculty of Veterinary Science in Budapest, Hungary in two periods 2009 and 2010. Results: Using the modified Knott's method, 33 dogs were positive for microfilariae (7.2%, 33/457) and using molecular technique, the following species were identified: D. repens (18/28) and D. immitis (1/28). Five samples (5/33) positive for microfilariae, not examined by PCR were examined by the Diro Speed® Test / Heartworm (Bio Veto Test, France).

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Background: Taking into consideration that dirofilariosis spreading in Romania, in this study we intended to determine the prevalence of Dirofilaria spp. infection in dogs from Timiș County. Methods: The epidemiological research was conducted between February 2008 and October 2010, on a total of 457 dogs. The modified Knott's method was used to identify the Dirofilaria spp. microfilariae. Blood samples positive for microfilariae were examined using the polymerase chain reaction (PCR), at the Szent Istvan University, Faculty of Veterinary Science in Budapest, Hungary in two periods 2009 and 2010. Results: Using the modified Knott's method, 33 dogs were positive for microfilariae (7.2%, 33/457) and using molecular technique, the following species were identified: D. repens (18/28) and D. immitis (1/28). Five samples (5/33) positive for microfilariae, not examined by PCR were examined by the Diro Speed® Test / Heartworm (Bio Veto Test, France).

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Available abstract

Background: Taking into consideration that dirofilariosis spreading in Romania, in this study we intended to determine the prevalence of Dirofilaria spp. infection in dogs from Timiș County. Methods: The epidemiological research was conducted between February 2008 and October 2010, on a total of 457 dogs. The modified Knott's method was used to identify the Dirofilaria spp. microfilariae. Blood samples positive for microfilariae were examined using the polymerase chain reaction (PCR), at the Szent Istvan University, Faculty of Veterinary Science in Budapest, Hungary in two periods 2009 and 2010. Results: Using the modified Knott's method, 33 dogs were positive for microfilariae (7.2%, 33/457) and using molecular technique, the following species were identified: D. repens (18/28) and D. immitis (1/28). Five samples (5/33) positive for microfilariae, not examined by PCR were examined by the Diro Speed® Test / Heartworm (Bio Veto Test, France).

Key concepts: Dirofilaria repens, Dirofilaria immitis, Veterinary medicine, Repens, Dirofilariasis, Dirofilaria, Biology, Medicine

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