EFFECT OF CAFFEINE ON FROG MUSCLE

Aim: The aim of the present investigation is to study the effects of caffeine on frog skeletal, cardiac and smooth muscle tissues. Material and methods: In this study muscle strips were obtained from Sartorius muscle, heart muscle and rectum of frog after pithing the animal. Caffeine (30mM) was administered in aqueous form with the help of Micropipette directly upon the isolated muscle sample. These isolated samples were prepared, fixed and analyzed with the help of laser beam where linear movement of muscle contraction is converted to angular movement which was recorded on the graph paper and further statistical analysis was done using student’s t-test to determine significance of differences between means. A “p” value of < 0.05 was considered significant. Conclusion: Caffeine (30mM) induces sustained contraction in skeletal and smooth muscle, but cardiac muscle does not respond to caffeine though it increases the natural beat of sinoatrial preparation. Keywords: Caffeine, laser beam, student’s t-test, pithing