2014•Unpublished venueRequires access

Structural studies of the exopolysaccharide produced by a submerged culture of entomopathogenic fungus Metarhizium anisopliae (Estudio estructural del exopolisacárido producido por un cultivo sumergido del hongo entomopatogénico Metarhizium anisopliae)

Luis Lillo, G. Cabello, Carlos L. Céspedes, A. Claudia, Jerónimo Pérez

Abstract

The exopolysaccharide (EPS) separated from the entomopathogenic fungus Metarhizium anisopliae was determined by gel permeation chromatography to be homogeneous. The high-performance anion-exchange chromatography with pulsed-amperometric detection (HPAE-PAD) showed a content of monosaccharides D-galactosamine and D-fucose at a molar ratio of about 2:1. The results obtained from Fourier transform-infrared spectroscopy (FT-IR) and second derivative FT-IR spectrum confirmed the proposed structure.

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Key concepts: Metarhizium anisopliae, Entomopathogenic fungus, Metarhizium, Monosaccharide, Locust, Gel permeation chromatography, Fourier transform infrared spectroscopy, Chromatography