ScholarLens Back to search
2005•Unpublished venueRequires access

Enhancer and Gene Trap Transposon Mutagenesis in Arabidopsis

Rob Martienssen, Patricia S. Springer

Open publisher page 2 citations

Abstract

Insertional mutagenesis in Arabidopsis can be conveniently accomplished either by Agrobacterium mediated TDNA transformation, or by transposable element mobilization. Recent advances have made T-DNA transformation one of the most efficient methods to generate large numbers of insertions in the Arabidopsis genome (1-3). Typically, several hundred independent T-DNA insertions can be generated per transformed plant. This is many more insertions than in most transposon schemes, but despite the labor involved, transposons have some important advantages:

About this research paper

What this paper is about

Insertional mutagenesis in Arabidopsis can be conveniently accomplished either by Agrobacterium mediated TDNA transformation, or by transposable element mobilization. Recent advances have made T-DNA transformation one of the most efficient methods to generate large numbers of insertions in the Arabidopsis genome (1-3). Typically, several hundred independent T-DNA insertions can be generated per transformed plant. This is many more insertions than in most transposon schemes, but despite the labor involved, transposons have some important advantages:

Why it matters

OpenAlex reports 2 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Insertional mutagenesis in Arabidopsis can be conveniently accomplished either by Agrobacterium mediated TDNA transformation, or by transposable element mobilization. Recent advances have made T-DNA transformation one of the most efficient methods to generate large numbers of insertions in the Arabidopsis genome (1-3). Typically, several hundred independent T-DNA insertions can be generated per transformed plant. This is many more insertions than in most transposon schemes, but despite the labor involved, transposons have some important advantages:

Key concepts: Insertional mutagenesis, Transposable element, Arabidopsis, Sleeping Beauty transposon system, Mutagenesis, Transfer DNA, Transposon mutagenesis, Genetics

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Enhancer and Gene Trap Transposon Mutagenesis in Arabidopsis — Research Paper | ScholarLens