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1976•Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)Open access

Studies on the Formation of Volatile Amines from Trimethylamine Oxide by γ-Irradiation (I)

Sadao UCHIYAMA, Tatsuo KONDO, Mitsuru Uchiyama

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Abstract

The simplified method for determination of ammonia and volatile amines, especially methylamines formed from trimethylamine oxide (TMAO) by γ irradiation, was established.The method involved the extraction procedure by means of Conway's micro diffusion apparatus and the determination by TLC-fluorodensitometry of DNS-derivatives as well as by gas-liquid chromatography.The satisfactory quantitative results were obtained using ammonia, monomethylamine and dimethylamine in the concentration of 1×10-7-3×10-6mol/ml and trimethylamine in the concentration of 8×10-8-1×10-5mol/ml.

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The simplified method for determination of ammonia and volatile amines, especially methylamines formed from trimethylamine oxide (TMAO) by γ irradiation, was established.The method involved the extraction procedure by means of Conway's micro diffusion apparatus and the determination by TLC-fluorodensitometry of DNS-derivatives as well as by gas-liquid chromatography.The satisfactory quantitative results were obtained using ammonia, monomethylamine and dimethylamine in the concentration of 1×10-7-3×10-6mol/ml and trimethylamine in the concentration of 8×10-8-1×10-5mol/ml.

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Available abstract

The simplified method for determination of ammonia and volatile amines, especially methylamines formed from trimethylamine oxide (TMAO) by γ irradiation, was established.The method involved the extraction procedure by means of Conway's micro diffusion apparatus and the determination by TLC-fluorodensitometry of DNS-derivatives as well as by gas-liquid chromatography.The satisfactory quantitative results were obtained using ammonia, monomethylamine and dimethylamine in the concentration of 1×10-7-3×10-6mol/ml and trimethylamine in the concentration of 8×10-8-1×10-5mol/ml.

Key concepts: Trimethylamine, Dimethylamine, Methylamines, Trimethylamine N-oxide, Chemistry, Ammonia, Chromatography, Oxide

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