1984•Journal of Economic EntomologyRequires access

Plastic-Bead ELISA: An Inexpensive Epidemiological Tool for Detecting Gypsy Moth (Lepidoptera: Lymantriidae) Nuclear Polyhedrosis Virus

Michael Ma, Joseph K. Burkholder, R. E. Webb, H. T. Hsu

Abstract

Nylon and polystyrene beads were used in an enzyme-linked immunosorbent assay (ELISA) developed for detecting gypsy moth, Lymantria dispar L., nuclear polyhedrosis virus. Potentially, plastic-bead ELISA is an inexpensive and specific method that can be used for the quantitative measurement of insect pathogens and other proteins of interest.

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Key concepts: Lymantria dispar, Gypsy moth, Nuclear Polyhedrosis Virus, Lepidoptera genitalia, Biology, Baculoviridae, Virology, Bead