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1979•Journal of GerontologyRequires access

Trypsinization Frequency and Loss of Proliferative Capacity in WI-38 Cells

E. C. Hadley, E. D. Kress, V. J. Cristofalo

Open publisher page 16 citations

Abstract

The effect of the trypsinization procedure employed in routine serial subcultivation on the proliferative life span of WI-38 cells was studied by varying the frequency of trypsinization and by using a nonenzymatic subcultivation technique. The results indicate that recurrent trypsinization does not contribute to loss of proliferative capacity and may, in fact, delay this loss to some degree.

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What this paper is about

The effect of the trypsinization procedure employed in routine serial subcultivation on the proliferative life span of WI-38 cells was studied by varying the frequency of trypsinization and by using a nonenzymatic subcultivation technique. The results indicate that recurrent trypsinization does not contribute to loss of proliferative capacity and may, in fact, delay this loss to some degree.

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OpenAlex reports 16 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

The effect of the trypsinization procedure employed in routine serial subcultivation on the proliferative life span of WI-38 cells was studied by varying the frequency of trypsinization and by using a nonenzymatic subcultivation technique. The results indicate that recurrent trypsinization does not contribute to loss of proliferative capacity and may, in fact, delay this loss to some degree.

Key concepts: Trypsinization, Life span, Chemistry, Trypsin, Biology, Medicine, Biochemistry, Gerontology

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