Preparation of a collagen I-sodium hyaluronate-fibrin glue tri-copolymer scaffold bonding with antigen-extracted bovine cancellous bone and its characteristics.

[Objective]To prepare and investigate a scaffold made of collagenⅠ-sodium hyaluronate-fibrin glue tri-copolymer bonding with antigen-extracted bovine cancellous bone in vitro. [Methods]The osteochondral scaffold was constructed of collagenⅠ-sodium hyaluronate-fibrin glue tri-copolymer scaffold and antigen-extracted bovine cancellous bone with adhesion of the two parts by fibrin glue.Pore size and internal pores of the osteochondral scaffold were observed by gross and scanning electron microscopy(SEM).Rabbit bone marrow stromal cells(BMSCs) were isolated and amplified before they were seeded into the scaffold at the third passage.The proliferation of the cells on the scaffolds was examined using MTT method and the growth curve was drawn.The scaffolds with cells were dehydrated and observed by SEM after 7 days.[Results]The osteochondral scaffold after crosslinking and adhesion had the porous structure.The bone scaffold was bonded to the cartilage scaffold solidly.The examination under SEM indicated that the BMSCs adhered and spread well on the scaffold,and the extracellular matrices were also observed around the BMSCs.MTT method revealed that there was no significant difference between the experimental group and the control group at each time point(P0.05).[Conclusion]The osteochondral scaffold of the collagenⅠ-sodium hyaluronate-fbrin glue tri-copolymer scaffold bonding with antigen-extracted bovine cancellous bone has an appropriate structure and a good biocompatibility,which makes it a useful scaffold in the osteochondral tissue engineering.