In vitro introduction adventitious shoots and plant regeneration of sengon (Paraserianthes falcataria (L.) Nielsen)

Adventitious buds were obtained from isolated cotyledons cultured on MS medium with various concentrations of 6-benzylamino purine (BA) and thidiazuron (TDZ).The highest numbers of adventitious buds were obtained on MS medium supplemented with 0.2 mg/L BA.Experimental culturing with half the petiole portion and half with the terminal segments were grown on MS medium contained with 0.2 mg/L BA.Frequency of the adventitious bud induction was variable accordingly to the type of cultured explants.Explants with the half petiole showed the highest adventitious bud induction rate (80%) compared to explants of half with terminal segment (20%).An elongated shoot from the buds and growth of advent roots were both possible on the 1/2 MS medium without a plant growth regulator.These results offer an effective way in which clonal propagation can be accomplished.