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Heat Stability of Avidin and Avidin-Biotin Complex and Influence of Ionic Strength on Affinity of Avidin for Biotin.

R.-D. Wei, Lemuel D. Wright

Open publisher page 20 citations

Abstract

An assay method involving the use of radioactive biotin and Sephadex chromatography was used to determine avidin and/or the avidin-biotin complex. It was demonstrated that avidin is relatively heat labile while the avidin-biotin complex is relatively heat stable. It was found that the affinity of avidin for biotin, essentially zero in aqueous solution, is a direct function of the ionic strength of the medium in which avidin and biotin react.

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What this paper is about

An assay method involving the use of radioactive biotin and Sephadex chromatography was used to determine avidin and/or the avidin-biotin complex. It was demonstrated that avidin is relatively heat labile while the avidin-biotin complex is relatively heat stable. It was found that the affinity of avidin for biotin, essentially zero in aqueous solution, is a direct function of the ionic strength of the medium in which avidin and biotin react.

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Available abstract

An assay method involving the use of radioactive biotin and Sephadex chromatography was used to determine avidin and/or the avidin-biotin complex. It was demonstrated that avidin is relatively heat labile while the avidin-biotin complex is relatively heat stable. It was found that the affinity of avidin for biotin, essentially zero in aqueous solution, is a direct function of the ionic strength of the medium in which avidin and biotin react.

Key concepts: Avidin, Biotin, Chemistry, Biotinylation, Ionic strength, Chromatography, Biochemistry, Aqueous solution

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Heat Stability of Avidin and Avidin-Biotin Complex and Influence of Ionic Strength on Affinity of Avidin for Biotin. — Research Paper | ScholarLens