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Dimethyl Sulfoxide As a Protective Agent During Freezing and Thawing of Human Spermatozoa.

J.K. Sherman

Open publisher page 31 citations

Abstract

Dimethyl sulfoxide (DMSO) was evaluated, in terms of motility, as a freeze-thaw protective agent with human spermatozoa. DMSO was found to be equal to glycerol in this protective capacity but proved more toxic during storage at 22°C both before freezing and after thawing. An interaction of protective and toxic effects was noted in a comparison of 5, 10, 15 and 20% DMSO which suggested the 10% level as the most favorable. On the basis of findings, DMSO is not recommended as a substitute for glycerol in the preservation of human spermatozoa by freezing with the methods described.

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What this paper is about

Dimethyl sulfoxide (DMSO) was evaluated, in terms of motility, as a freeze-thaw protective agent with human spermatozoa. DMSO was found to be equal to glycerol in this protective capacity but proved more toxic during storage at 22°C both before freezing and after thawing. An interaction of protective and toxic effects was noted in a comparison of 5, 10, 15 and 20% DMSO which suggested the 10% level as the most favorable. On the basis of findings, DMSO is not recommended as a substitute for glycerol in the preservation of human spermatozoa by freezing with the methods described.

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Available abstract

Dimethyl sulfoxide (DMSO) was evaluated, in terms of motility, as a freeze-thaw protective agent with human spermatozoa. DMSO was found to be equal to glycerol in this protective capacity but proved more toxic during storage at 22°C both before freezing and after thawing. An interaction of protective and toxic effects was noted in a comparison of 5, 10, 15 and 20% DMSO which suggested the 10% level as the most favorable. On the basis of findings, DMSO is not recommended as a substitute for glycerol in the preservation of human spermatozoa by freezing with the methods described.

Key concepts: Dimethyl sulfoxide, Glycerol, Cryopreservation, Chemistry, Motility, Sulfoxide, Andrology, Biochemistry

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