1954•Poultry ScienceOpen access
Response of Kittens to the California Strain of Newcastle Disease Virus (NDV) After Oral and Nasal Routes of Exposure
Reginald L. Reagan, Edward C. Delaha, Sue R. Cook, Arthur L. Brueckner
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Abstract
The egg-adapted Newcastle disease virus (NDV) California strain used for this study was obtained in 1954 from the Bureau of Animal Industry, Department of Agriculture, Washington, D. C. This strain was isolated by the Bureau from infected chicken tissue from the corresponding state before the introduction of commercial live virus vaccines for Newcastle disease. The culture had been carried in 10-day embryonated chicken eggs through 28 serial passages. Embryo passage of the California strain was continued through the 29th passage. Allanto-amniotic fluid from several eggs dead of the strain was harvested and the strain of the virus was confirmed to be NDV by a neutralization test conducted in 10-day embryonated chicken eggs. The test was performed according to the procedure recommended by the Bureau of Animal Industry (U. S. Dept. Agri. Bull., 1946). Newcastle-immune chicken serum neutralized the virus in all cases while normal chicken serum had no effect. Reagan, …